Selected article for: "fold serial dilution and serial dilution"
Author: Shefali Dobhal; Gamze Boluk; Brooke Babler; Michael J. Stulberg; John Rascoe; Mark Nakhla; Toni A. Chapman; Alex B. Crockford; Michael Melzer; Anne M. Alvarez; Mohammad Arif
Title: Comparative genomics reveals signature regions used to develop a robust and sensitive multiplex TaqMan real-time qPCR assay to detect the genus Dickeya and Dickeya dianthicola
  • Document date: 2019_11_20
    • ID: lgeu4id0_15
    Snippet: To compare the sensitivity of the multiplex with the single TaqMan qPCR assay, the ten-fold serially diluted DNA was used with the single flap primer set. Two separate reaction mixes were prepared, one containing the primer set targeting the genus Dickeya and the other targeting D. dianthicola. The ten-fold serial dilution sensitivity was performed in the similar way as described above......
    Document: To compare the sensitivity of the multiplex with the single TaqMan qPCR assay, the ten-fold serially diluted DNA was used with the single flap primer set. Two separate reaction mixes were prepared, one containing the primer set targeting the genus Dickeya and the other targeting D. dianthicola. The ten-fold serial dilution sensitivity was performed in the similar way as described above.

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