Author: Ma, Ge; Greenwell-Wild, Teresa; Lei, Kejian; Jin, Wenwen; Swisher, Jennifer; Hardegen, Neil; Wild, Carl T.; Wahl, Sharon M.
Title: Secretory Leukocyte Protease Inhibitor Binds to Annexin II, a Cofactor for Macrophage HIV-1 Infection Document date: 2004_11_15
ID: rlabxfss_9
Snippet: Nano-Capillary HPLC Ion Trap Mass Spectrometry (LC-MS/MS). Membrane protein fractions from monocyte (2-10 ϫ 10 9 ) or macrophage (2-10 ϫ 10 9 ) coimmunoprecipitations were pooled and separated on SDS-PAGE. The gels were stained with colloidal blue, destained, photographed, and the desired protein band(s) was marked before storage at 4 Њ C. The gels were analyzed with LC-MS/MS of in-gel tryptic digests for subsequent protein identification by d.....
Document: Nano-Capillary HPLC Ion Trap Mass Spectrometry (LC-MS/MS). Membrane protein fractions from monocyte (2-10 ϫ 10 9 ) or macrophage (2-10 ϫ 10 9 ) coimmunoprecipitations were pooled and separated on SDS-PAGE. The gels were stained with colloidal blue, destained, photographed, and the desired protein band(s) was marked before storage at 4 Њ C. The gels were analyzed with LC-MS/MS of in-gel tryptic digests for subsequent protein identification by database searching (Wistar Institute). The output from the National Center for Biotechnology Information and nonredundant database search using SEQUEST consists of peptide sequences matched to protein(s) in the database searched by correlated predicted and observed peptide fragmentation patterns.
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