Author: Zheng, Li-Zhen; Wang, Jia-Li; Kong, Ling; Huang, Le; Tian, Li; Pang, Qian-Qian; Wang, Xin-Luan; Qin, Ling
Title: Steroid-associated osteonecrosis animal model in rats Document date: 2018_2_6
ID: tad9jmfp_19
Snippet: The proximal and distal femora and proximal and distal tibiae were decalcified by 10% EDTA (pH 7.4), embedded in paraffin and cut into 5-mmethick sections along the coronal plane. The livers were fixed in buffered formalin, embedded in paraffin and cut into 5-mmethick sections. Sections were stained with haematoxylin and eosin (H&E), and tartrate-resistant acid phosphatase staining (Sigma Diagnostics, Missouri, USA) was performed for osteoclast a.....
Document: The proximal and distal femora and proximal and distal tibiae were decalcified by 10% EDTA (pH 7.4), embedded in paraffin and cut into 5-mmethick sections along the coronal plane. The livers were fixed in buffered formalin, embedded in paraffin and cut into 5-mmethick sections. Sections were stained with haematoxylin and eosin (H&E), and tartrate-resistant acid phosphatase staining (Sigma Diagnostics, Missouri, USA) was performed for osteoclast accounts. The Microfil-perfused tibiae were paraffin sectioned, and the vascular endothelial growth factor (VEGF) expression in bone marrow was tested by immunohistochemistry with antibody VEGF (sc-7269) (SC; Santa Cruz, CA, USA).
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