Author: Peña, José; Chen-Harris, Haiyin; Allen, Jonathan E.; Hwang, Mona; Elsheikh, Maher; Mabery, Shalini; Bielefeldt-Ohmann, Helle; Zemla, Adam T.; Bowen, Richard A.; Borucki, Monica K.
Title: Sendai virus intra-host population dynamics and host immunocompetence influence viral virulence during in vivo passage Document date: 2016_4_9
ID: z7f720dj_34
Snippet: Quantitative PCR was used to measure expression of host cytokine genes in lung homogenate using Taqman Gene Expression Assays (Life Technologies) for the mouse cytokines and QuantiTect SYBR Green PCR (Invitrogen) for the guinea pig cytokines. Total RNA was extracted using TRIzol LS Reagent (Invitrogen) following the manufacturer's protocol. Reverse transcription was performed using Oligo dT primers and the Superscript III RT reverse transcriptase.....
Document: Quantitative PCR was used to measure expression of host cytokine genes in lung homogenate using Taqman Gene Expression Assays (Life Technologies) for the mouse cytokines and QuantiTect SYBR Green PCR (Invitrogen) for the guinea pig cytokines. Total RNA was extracted using TRIzol LS Reagent (Invitrogen) following the manufacturer's protocol. Reverse transcription was performed using Oligo dT primers and the Superscript III RT reverse transcriptase kit (Invitrogen) following the manufacturer's protocol. Primer sequences for amplification of guinea pig mRNA were obtained from Scott and Aronson (2008) and Jeevan et al. (2011) , GAPDH was used as endogenous control for both the mouse and guinea pig cytokine measurements.
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