Selected article for: "International license and PCR assay"
Author: Fan Wu; Su Zhao; Bin Yu; Yan-Mei Chen; Wen Wang; Yi Hu; Zhi-Gang Song; Zhao-Wu Tao; Jun-Hua Tian; Yuan-Yuan Pei; Ming-Li Yuan; Yu-Ling Zhang; Fa-Hui Dai; Yi Liu; Qi-Min Wang; Jiao-Jiao Zheng; Lin Xu; Edward C. Holmes; Yong-Zhen Zhang
Title: Complete genome characterisation of a novel coronavirus associated with severe human respiratory disease in Wuhan, China
  • Document date: 2020_1_25
    • ID: 5jai745w_13
    Snippet: As the longest contigs generated by Megahit (30,474 nt) and Trinity (11,760 nt) both 351 had high similarity to the bat SARS-like coronavirus isolate bat-SL-CoVZC45 and were at 352 high abundance (Table S1 and S2), the longer one (30,474 nt) that covered almost the whole 353 virus genome was used for primer design for PCR confirmation and genome termini 354 determination. Primers used in PCR, qPCR and RACE experiments are listed in Table S8 . The.....
    Document: As the longest contigs generated by Megahit (30,474 nt) and Trinity (11,760 nt) both 351 had high similarity to the bat SARS-like coronavirus isolate bat-SL-CoVZC45 and were at 352 high abundance (Table S1 and S2), the longer one (30,474 nt) that covered almost the whole 353 virus genome was used for primer design for PCR confirmation and genome termini 354 determination. Primers used in PCR, qPCR and RACE experiments are listed in Table S8 . The 355 PCR assay was conducted as described previously 9 and the complete genome termini was Table S1 . The top 50 abundant assembled contigs generated using the Megahit program. Figure S1 . Detection of other respiratory pathogens by qPCR. The copyright holder for this preprint (which was not peer-reviewed) is the . https: //doi.org/10.1101 //doi.org/10. /2020 . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.01.24.919183 doi: bioRxiv preprint

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