Author: Stewart, Meredith E.; Roy, Polly
Title: Structure-based identification of functional residues in the nucleoside-2'-O-methylase domain of Bluetongue virus VP4 capping enzyme Document date: 2015_2_24
ID: vzel6r43_12
Snippet: Synthesis of capped and uncapped BTV transcripts was as described previously [3, 19] . Briefly, capped T7 transcripts were synthesized by using a mMESSAGE mMACHINE T7 Ultra Kit (Ambion), while uncapped T7 transcripts were synthesized by the RiboMAX Large-Scale RNA Production System T7 (Promega) according to the manufacturer's procedure. a 32 P-labelled RNA was synthesized using 2.5 mM GTP and 50 lCi a 32 P-GTP. All RNA transcripts were dissolved .....
Document: Synthesis of capped and uncapped BTV transcripts was as described previously [3, 19] . Briefly, capped T7 transcripts were synthesized by using a mMESSAGE mMACHINE T7 Ultra Kit (Ambion), while uncapped T7 transcripts were synthesized by the RiboMAX Large-Scale RNA Production System T7 (Promega) according to the manufacturer's procedure. a 32 P-labelled RNA was synthesized using 2.5 mM GTP and 50 lCi a 32 P-GTP. All RNA transcripts were dissolved in nuclease-free water and stored at À80°C.
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