Author: Angelini, Megan M.; Akhlaghpour, Marzieh; Neuman, Benjamin W.; Buchmeier, Michael J.
Title: Severe Acute Respiratory Syndrome Coronavirus Nonstructural Proteins 3, 4, and 6 Induce Double-Membrane Vesicles Document date: 2013_8_13
ID: yl1qyh7j_23
Snippet: Percentages found in Table 1 are based on the raw number of cells counted that contained a given phenotype compared to total number of cells counted. Table 2 compares the observed frequency of nsp-related intracellular features to the expected frequency based on the size of the feature relative to the size of the cell and the number of plasmids required to produce the feature. The estimate assumes an independent 70% transfection rate for each pla.....
Document: Percentages found in Table 1 are based on the raw number of cells counted that contained a given phenotype compared to total number of cells counted. Table 2 compares the observed frequency of nsp-related intracellular features to the expected frequency based on the size of the feature relative to the size of the cell and the number of plasmids required to produce the feature. The estimate assumes an independent 70% transfection rate for each plasmid and an average cell diameter of 15 m. Expected frequencies were calculated as transfection efficiency times the ratio of feature size to cell size. Expected frequencies were summed for combinations that would produce the same feature.
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