Selected article for: "real time and Trizol reagent"
Author: Blank, Maximilian F.; Chen, Sifan; Poetz, Fabian; Schnölzer, Martina; Voit, Renate; Grummt, Ingrid
Title: SIRT7-dependent deacetylation of CDK9 activates RNA polymerase II transcription
  • Document date: 2017_3_17
    • ID: qm9urt2w_12
    Snippet: Cellular RNA was isolated with TRIzol Reagent (Invitrogen), transcribed into cDNA using random primers (Roche), and analyzed by real-time PCR (Roche, LightCy-cler480). Radiolabeled RNA was generated by in vitro transcription using the MEGAscript T7 Transcription Kit (Ambion) and templates generated by PCR with gene-specific primers fused to the T7 promoter. Primers are listed in Supplementary Table S1 ......
    Document: Cellular RNA was isolated with TRIzol Reagent (Invitrogen), transcribed into cDNA using random primers (Roche), and analyzed by real-time PCR (Roche, LightCy-cler480). Radiolabeled RNA was generated by in vitro transcription using the MEGAscript T7 Transcription Kit (Ambion) and templates generated by PCR with gene-specific primers fused to the T7 promoter. Primers are listed in Supplementary Table S1 .

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