Author: te Velthuis, Aartjan J.W.; van den Worm, Sjoerd H. E.; Snijder, Eric J.
Title: The SARS-coronavirus nsp7+nsp8 complex is a unique multimeric RNA polymerase capable of both de novo initiation and primer extension Document date: 2011_10_29
ID: tx0lqgff_31
Snippet: Given nsp(7+8)'s ability to bind dsRNA, we wondered whether this protein complex would also be catalytically active on this type of template and able to incorporate nucleoside monophosphates (NMPs) into partially double-stranded RNA molecules, i.e. primed templates. We therefore examined the ability of nsp8 to extend a 20-nt primer that was pre-annealed to a heteromeric template with relatively low secondary structure, to rule out potential adver.....
Document: Given nsp(7+8)'s ability to bind dsRNA, we wondered whether this protein complex would also be catalytically active on this type of template and able to incorporate nucleoside monophosphates (NMPs) into partially double-stranded RNA molecules, i.e. primed templates. We therefore examined the ability of nsp8 to extend a 20-nt primer that was pre-annealed to a heteromeric template with relatively low secondary structure, to rule out potential adverse effects of hairpins ( Figure 4A ). Interestingly and in contrast to previous observations (12) , the nsp(7+8) complex readily extended the primer up to template length, resulting in the formation of a 40-base pair RNA duplex ( Figure 4B ).
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