Title: The signal sequence of the p62 protein of Semliki Forest virus is involved in initiation but not in completing chain translocation Document date: 1990_9_1
ID: rmjv56ia_22
Snippet: We first tested the expression of in vitro-made RNA from the construction pCp62dhfr in an in vitro translation system. This would be expected to yield free C protein and p62reporter hybrid (p62-dhfr) through C-catalyzed autoproteolytic cleavage of the nascent C-p62-reporter precursor ( Fig. 1 ) (Aliperti and Schlesinger, 1978; Hahn et al., 1985; Melancon and Garoff, 1987) . Furthermore, the p62-reporter hybrid should be translocated across micro.....
Document: We first tested the expression of in vitro-made RNA from the construction pCp62dhfr in an in vitro translation system. This would be expected to yield free C protein and p62reporter hybrid (p62-dhfr) through C-catalyzed autoproteolytic cleavage of the nascent C-p62-reporter precursor ( Fig. 1 ) (Aliperti and Schlesinger, 1978; Hahn et al., 1985; Melancon and Garoff, 1987) . Furthermore, the p62-reporter hybrid should be translocated across microsomal membranes and possibly glycosylated at Asn~3 of the p62 sequence if the 40 residues long NH2-terminal p62 peptide carries a signal sequence.
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