The signal sequence of the p62 protein of Semliki Forest virus is involved in initiation but not in completing chain translocation

The samples were treated, after translation, at pH 11-11.5, and the proteins then separated into a membrane-bound pellet fraction (P) and a supernatant fraction (S) by centrifugation. In all samples the p62-dhfr polypeptides were isolated using an anti-dhfr antibody. The proteins were then analyzed by SDS-PAGE (10 %) and subsequent autoradiography. The slower migrating band corresponds to glycosylated and the faster one to nonglycosylated forms of p62-dhfr (compare Fig. 3) . tion/translation mixtures involving cDNAs with C regions (compare Fig. 6 ).