The signal sequence of the p62 protein of Semliki Forest virus is involved in initiation but not in completing chain translocation

To see whether the C protein exerts an influence on the translation phenotype of the p62-dhfr protein the p62dhfr plasmid (see Fig. 1 ), lacking the C gene, was tested. The results shown in Fig. 4 show clearly that the p62-dhfr hybrid is translocated and glycosylated in the same way as when expressed from pCp62dhfr. Thus, apart from providing a free NH2-terminal end to the p62-dhfr protein by autoproteolysis of the C-p62-dhfr precursor the C protein has no role in the translocation process.