Title: The single transmembrane segment of gp210 is sufficient for sorting to the pore membrane domain of the nuclear envelope Document date: 1992_12_2
ID: vznqgnzd_16
Snippet: Expression of the wild-type CD8 eDNA was accomplished as follows. A pSP65 plasmid containing the F1 eDNA clone of CD8 (18) (pSP65F1, kindly provided by Dr. Giovanni Migliaccio, Istituto di Ricerehe di Biologia Molecolare, Pomezia, Italy) was digested with EcoRI to remove the fulllength CD8 eDNA. The cohesive ends were filled in with Klenow fragment and the eDNA was subeloned into the Sinai site of the pSVL plasmid for expression studies......
Document: Expression of the wild-type CD8 eDNA was accomplished as follows. A pSP65 plasmid containing the F1 eDNA clone of CD8 (18) (pSP65F1, kindly provided by Dr. Giovanni Migliaccio, Istituto di Ricerehe di Biologia Molecolare, Pomezia, Italy) was digested with EcoRI to remove the fulllength CD8 eDNA. The cohesive ends were filled in with Klenow fragment and the eDNA was subeloned into the Sinai site of the pSVL plasmid for expression studies.
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