Author: Klaile, Esther; Vorontsova, Olga; Sigmundsson, Kristmundur; Müller, Mario M.; Singer, Bernhard B.; Öfverstedt, Lars-Göran; Svensson, Stina; Skoglund, Ulf; Öbrink, Björn
Title: The CEACAM1 N-terminal Ig domain mediates cis- and trans-binding and is essential for allosteric rearrangements of CEACAM1 microclusters Document date: 2009_11_16
ID: uy2553z7_31
Snippet: Although homophilically binding proteins have been analyzed by flow cell biosensor techniques (Korotkova et al., 2008) , it has previously not been possible to analyze the kinetics of such a system because no algorithms have existed that can deal with a situation in which the same binding interactions take place both within the mobile phase and between the mobile and solid phases. However, the novel curve-fitting algorithms that we present in thi.....
Document: Although homophilically binding proteins have been analyzed by flow cell biosensor techniques (Korotkova et al., 2008) , it has previously not been possible to analyze the kinetics of such a system because no algorithms have existed that can deal with a situation in which the same binding interactions take place both within the mobile phase and between the mobile and solid phases. However, the novel curve-fitting algorithms that we present in this study allowed us to identify two different reactions that resulted in the formation of two types of dimers and one type of trimer in the presence of divalent cations. The equilibrium dissociation constants that were obtained from the curve fitting were in good agreement with the equilibrium dissociation constants determined from the tomography data. Both methods gave constants that were of the same order of magnitude for either type of dimerization reaction and showed the same dependence on divalent cations. These findings strongly support the interpretations of both the SPR-binding analyses and the molecular electron tomography.
Search related documents:
Co phrase search for related documents- bind interaction and equilibrium dissociation: 1
- bind interaction and equilibrium dissociation constant: 1
- bind interaction and good agreement: 1
- bind interaction and SPR bind: 1
- bind interaction and study present: 1, 2, 3
- bind protein and dissociation constant: 1, 2, 3, 4, 5
- bind protein and equilibrium dissociation: 1, 2, 3
- bind protein and equilibrium dissociation constant: 1, 2, 3
- bind protein and good agreement: 1, 2
- bind protein and SPR bind: 1, 2
- bind protein and study present: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30
- bind protein and system kinetic: 1
- bind protein and trimer type: 1
- cell biosensor and divalent cation: 1
- cell biosensor and divalent cation dependence: 1
- cell biosensor and magnitude order: 1
- cell biosensor and study present: 1
Co phrase search for related documents, hyperlinks ordered by date