Selected article for: "account model and model fit"
Author: Klaile, Esther; Vorontsova, Olga; Sigmundsson, Kristmundur; Müller, Mario M.; Singer, Bernhard B.; Öfverstedt, Lars-Göran; Svensson, Stina; Skoglund, Ulf; Öbrink, Björn
Title: The CEACAM1 N-terminal Ig domain mediates cis- and trans-binding and is essential for allosteric rearrangements of CEACAM1 microclusters
  • Document date: 2009_11_16
    • ID: uy2553z7_55
    Snippet: One additional complication arises in the curve-fit routine for the trimer model based on the fact that, in contrast to a single form of a trimer in solution, the surface-bound trimers cannot be treated as equal. Because one of the three components belonging to the trimer will be the immobilized ligand, an asymmetry is introduced. Because of different responses for the association or dissociation of monomers versus dimers to a surface-bound ligan.....
    Document: One additional complication arises in the curve-fit routine for the trimer model based on the fact that, in contrast to a single form of a trimer in solution, the surface-bound trimers cannot be treated as equal. Because one of the three components belonging to the trimer will be the immobilized ligand, an asymmetry is introduced. Because of different responses for the association or dissociation of monomers versus dimers to a surface-bound ligand, together with different interaction rates for the two types of binding sites involved, the model has to account for two types of dimers and three types of trimers contributing to the SPR-based signal. These ligand complexes are distinguished as R AL , R LA , R AAL , R LAA , and R ALA (Fig. S1 ). As for the other two models, this model has to be provided with information on A Total (in grams/ liter), M W , R max , and initial values for the rate constants. Finally, together with Eq. 3.5 for the estimation of [A B ], Eqs. 3.2 and 3.3 for the estimation of the dimer [ A B 2 ] forms and Eq. 3.4 for the estimation of the

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