Author: Wang, Yi; Liu, Li
Title: The Membrane Protein of Severe Acute Respiratory Syndrome Coronavirus Functions as a Novel Cytosolic Pathogen-Associated Molecular Pattern To Promote Beta Interferon Induction via a Toll-Like-Receptor-Related TRAF3-Independent Mechanism Document date: 2016_2_9
ID: uf96jgig_11
Snippet: The SARS-CoV M gene product functions at the protein level to induce IFN-⤠production. The next question that we tried to ask was at which level (mRNA or protein) the M-mediated IFN-⤠induction occurred. To address this issue directly, we created an M-stop construct by replacing the start codon AUG with three in-frame tandem stop codons at the 5= end of the M gene (Fig. 5A ). This expression construct can generate only mRNA and no protein due.....
Document: The SARS-CoV M gene product functions at the protein level to induce IFN-⤠production. The next question that we tried to ask was at which level (mRNA or protein) the M-mediated IFN-⤠induction occurred. To address this issue directly, we created an M-stop construct by replacing the start codon AUG with three in-frame tandem stop codons at the 5= end of the M gene (Fig. 5A ). This expression construct can generate only mRNA and no protein due to the translation failure of the mRNA substrates. Western blot analysis shows that the M protein synthesis was completely blocked in the M-stop construct but not the wild-type M construct (Fig. 5B ). Real-time quantitative reverse transcription PCR (qRT-PCR) analysis shows that the M-stop construct did not induce IFN-⤠production in HEK293T cells, indicating that M-mediated IFN-⤠production is dependent on M protein rather than M mRNA (Fig. 5C) .
Search related documents:
Co phrase search for related documents- blot analysis and expression construct: 1, 2, 3, 4, 5, 6, 7
- blot analysis and issue address: 1
- blot analysis and mrna level: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15
- blot analysis and mrna level protein: 1, 2
- blot analysis and production induce: 1, 2, 3
- blot analysis and protein level: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21
- blot analysis and protein synthesis: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
- blot analysis and qrt PCR analysis: 1, 2, 3, 4, 5
- blot analysis and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46
- blot analysis and western blot analysis: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84
- blot analysis and wild type: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27
- codon stop and issue address: 1, 2
- codon stop and mRNA generate: 1, 2
- codon stop and protein level: 1, 2
- codon stop and protein synthesis: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18
- codon stop and real time: 1, 2
- codon stop and wild type: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
Co phrase search for related documents, hyperlinks ordered by date