Title: The organization of endoplasmic reticulum export complexes Document date: 1996_10_1
ID: xxlcdbqi_22
Snippet: Buds in Export Complexes. The local density of buds on individual cisternae associated with export complexes was determined as follows: using a stack of sequential serial sections, we followed one continuous bud-bearing zone of ER membrane that contained at least four buds. The distances between the most distant ER buds in the stack were directly measured in both the plane of the section and the depth of the stack. These two distances were multip.....
Document: Buds in Export Complexes. The local density of buds on individual cisternae associated with export complexes was determined as follows: using a stack of sequential serial sections, we followed one continuous bud-bearing zone of ER membrane that contained at least four buds. The distances between the most distant ER buds in the stack were directly measured in both the plane of the section and the depth of the stack. These two distances were multiplied by one another to get a surface area of the plane of the ER bud-bearing region. The total number of ER buds in such a zone (obtained by reconstruction as described above for the Golgi exclusion zone) was then divided by the area of the bud-bearing zone to determine a local ER bud surface density.
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