Author: Hao, Xin-yan; Lv, Qi; Li, Feng-di; Xu, Yan-feng; Gao, Hong
Title: The characteristics of hDPP4 transgenic mice subjected to aerosol MERS coronavirus infection via an animal nose-only exposure device Document date: 2019_10_30
ID: vkjcheaz_18
Snippet: MERS-CoV virus suspensions (10 6.5 TCID 50 ) and serum-free Dulbecco's Modified Eagle Medium (DMEM) were separately added to the nebulizer reservoir to infect exposed mice in the aerosol and control aerosol groups, respectively, for 30 minutes. According to the instructions of the exposure device and mouse respiratory rate (25 mL/min per mouse), the nebulizer flow rate was set to 0.24 L/ min, the diluter flow rate was set to 6.8 L/min, and the ne.....
Document: MERS-CoV virus suspensions (10 6.5 TCID 50 ) and serum-free Dulbecco's Modified Eagle Medium (DMEM) were separately added to the nebulizer reservoir to infect exposed mice in the aerosol and control aerosol groups, respectively, for 30 minutes. According to the instructions of the exposure device and mouse respiratory rate (25 mL/min per mouse), the nebulizer flow rate was set to 0.24 L/ min, the diluter flow rate was set to 6.8 L/min, and the nebulizer pressure was set to 20 PSI. Mice were anesthetized with 1.2% tribromoethanol (0.2 mL/10 g of body weight, intramuscular (im)) for intranasal inoculation with 10 6.5 TCID 50 of MERS-CoV in the instillation group and serum-free DMEM in the instillation control group.
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