Selected article for: "cell line and dmem medium"
Author: Teoh, Kim-Tat; Siu, Yu-Lam; Chan, Wing-Lim; Schlüter, Marc A.; Liu, Chia-Jen; Peiris, J. S. Malik; Bruzzone, Roberto; Margolis, Benjamin; Nal, Béatrice
Title: The SARS Coronavirus E Protein Interacts with PALS1 and Alters Tight Junction Formation and Epithelial Morphogenesis
  • Document date: 2010_11_15
    • ID: ufw13pjx_62
    Snippet: In these experiments, suspensions of predominantly single cells of the specified cell lines were embedded into DMEM medium supplemented with 4% GelTrex on a thin bottom of solid GelTrex, and the cells were maintained at 37°C with 5% CO2 for 5 d to form cysts. Then, the cysts were fixed, permeabilized, and colabeled with appropriate antibody combinations, followed by confocal microscopy. To perform a phenotypic cyst assay, we have counted 870–8.....
    Document: In these experiments, suspensions of predominantly single cells of the specified cell lines were embedded into DMEM medium supplemented with 4% GelTrex on a thin bottom of solid GelTrex, and the cells were maintained at 37°C with 5% CO2 for 5 d to form cysts. Then, the cysts were fixed, permeabilized, and colabeled with appropriate antibody combinations, followed by confocal microscopy. To perform a phenotypic cyst assay, we have counted 870–885 cysts for each cell line and the phenotypes observed were categorized into cysts with either a single apical lumen or multiple lumens. Subsequently, a Student's t test was used to analyze whether defect of cysts morphogenesis significantly correlated with ectopical expression of E protein.

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