Selected article for: "affinity chromatography and SDS PAGE analysis"
Author: Kopertekh, Lilya; Meyer, Torsten; Freyer, Cornelia; Hust, Michael
Title: Transient plant production of Salmonella Typhimurium diagnostic antibodies
  • Document date: 2019_2_12
    • ID: y47ahl3p_29
    Snippet: Purification of scFv-TM43-E10 antibody fragment was performed by IMAC based on C-terminal His-tag. The scFv-Fc version of TM43-E10 antibody was purified from leaf extracts via Protein A affinity chromatography. SDS-PAGE analysis of the eluted fractions after affinity chromatography revealed that scFv-TM43-E10 and scFv-Fc-TM43-E10 proteins were selectively enriched from leaf extracts. Samples derived from the first purification step were separated.....
    Document: Purification of scFv-TM43-E10 antibody fragment was performed by IMAC based on C-terminal His-tag. The scFv-Fc version of TM43-E10 antibody was purified from leaf extracts via Protein A affinity chromatography. SDS-PAGE analysis of the eluted fractions after affinity chromatography revealed that scFv-TM43-E10 and scFv-Fc-TM43-E10 proteins were selectively enriched from leaf extracts. Samples derived from the first purification step were separated by size exclusion chromatography. Reducing SDS-PAGE analysis of the purified antibodies demonstrated single bands at approximately 28 kDa and 50 kDa for scFv-TM43-E10 and scFv-Fc-TM43-E10, respectively (Fig. 6) .

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