Author: Kopertekh, Lilya; Meyer, Torsten; Freyer, Cornelia; Hust, Michael
Title: Transient plant production of Salmonella Typhimurium diagnostic antibodies Document date: 2019_2_12
ID: y47ahl3p_31
Snippet: To determine the antigen detection limit ELISA plates were coated with different concentrations of OmpD protein ranging from 0.1 ng/ml to 1000 ng/ml and probed with purified plantproduced antibody fragments at a half maximal saturation. The results of this assay are illustrated in Fig. 6b . The minimal OmpD concentration that has been detected by scFv-TM43-E10 proteins was 50 ng/ml. The antigen detection limit of the scFv-Fc-TM43-E10 antibody fra.....
Document: To determine the antigen detection limit ELISA plates were coated with different concentrations of OmpD protein ranging from 0.1 ng/ml to 1000 ng/ml and probed with purified plantproduced antibody fragments at a half maximal saturation. The results of this assay are illustrated in Fig. 6b . The minimal OmpD concentration that has been detected by scFv-TM43-E10 proteins was 50 ng/ml. The antigen detection limit of the scFv-Fc-TM43-E10 antibody fragment was about 20 ng/ml.
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