Selected article for: "dna polymerase and PCR product"
Author: Kopertekh, Lilya; Meyer, Torsten; Freyer, Cornelia; Hust, Michael
Title: Transient plant production of Salmonella Typhimurium diagnostic antibodies
  • Document date: 2019_2_12
    • ID: y47ahl3p_8
    Snippet: In order to design the expression vector containing a PVX modified sequence and the gb gene silencing suppressor from Poa semilatent virus (PSLV) a gb amplicon was generated using SalI-BgP-forw and SpeI-BgP-rev primers and pPgb plasmid [33] as a template. The PCR product was cut with SalI and SpeI restriction enzymes and cloned into the XhoI-XbaI digested pRT103 plasmid generating the pRT-35S-gb-ter construct [34] . A 35S-gb-ter expression casset.....
    Document: In order to design the expression vector containing a PVX modified sequence and the gb gene silencing suppressor from Poa semilatent virus (PSLV) a gb amplicon was generated using SalI-BgP-forw and SpeI-BgP-rev primers and pPgb plasmid [33] as a template. The PCR product was cut with SalI and SpeI restriction enzymes and cloned into the XhoI-XbaI digested pRT103 plasmid generating the pRT-35S-gb-ter construct [34] . A 35S-gb-ter expression cassette was released from the pRT-35S-gb-ter plasmid by PstI and inserted into NsiI digested pLH-Dbar binary vector resulting in pLH-gb plasmid. The PVX-m sequence from pPVX-201m was digested with SphI-EheI, treated with T4 DNA polymerase and cloned into the StuI restricted pLH-gb plasmid yielding the pLH-gb-PVX-m transient expression vector.

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