Title: The signal sequence of the p62 protein of Semliki Forest virus is involved in initiation but not in completing chain translocation Document date: 1990_9_1
ID: rmjv56ia_18
Snippet: The two oligonucleotides were hybridized (51 complementary bases), filled in using Sequenase (United States Biochemical Co., Cleveland, OH) and restricted with Eco RI and Xho I. The resulting DNA fragment was then purified and inserted into pCp62dhfr instead of the C and p62 sequences. For this purpose the pCp62dhfr plasmid was Eco RI and Xho I restricted and the plasmid part with the dhfr sequences isolated. The resulting plasmid p62dhfr contain.....
Document: The two oligonucleotides were hybridized (51 complementary bases), filled in using Sequenase (United States Biochemical Co., Cleveland, OH) and restricted with Eco RI and Xho I. The resulting DNA fragment was then purified and inserted into pCp62dhfr instead of the C and p62 sequences. For this purpose the pCp62dhfr plasmid was Eco RI and Xho I restricted and the plasmid part with the dhfr sequences isolated. The resulting plasmid p62dhfr contains thus the coding sequences for the initiator methionine of C and the first 40 residues of the p62 protein, including the signal sequence, in front of the dhfr gene (see Fig. 1 ).
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