Author: Li, Dongbo; Ji, Hongfei; Niu, Xingjian; Yin, Lei; Wang, Yiran; Gu, Yucui; Wang, Jinlu; Zhou, Xiaoping; Zhang, Han; Zhang, Qingyuan
Title: Tumor-associated macrophages secrete CC-chemokine ligand 2 and induce tamoxifen resistance by activating PI3K/Akt/mTOR in breast cancer Document date: 2019_12_19
ID: yj9mb88g_7
Snippet: Cell proliferation was assessed using CCK-8 (Vazyme) assay according to the manufacturer's instructions. Briefly, the cells were seeded in 96-well plates at a density of 1 × 10 4 cells/well and incubated with 5 μmol/L tamoxifen for 0, 12, 24, 36, 48, 60, and 72 hours. At the K E Y W O R D S breast cancer, CCL2, tamoxifen resistance, tumor microenvironment, tumor-associated macrophage end of the respective incubation times, the cells were incuba.....
Document: Cell proliferation was assessed using CCK-8 (Vazyme) assay according to the manufacturer's instructions. Briefly, the cells were seeded in 96-well plates at a density of 1 × 10 4 cells/well and incubated with 5 μmol/L tamoxifen for 0, 12, 24, 36, 48, 60, and 72 hours. At the K E Y W O R D S breast cancer, CCL2, tamoxifen resistance, tumor microenvironment, tumor-associated macrophage end of the respective incubation times, the cells were incubated in complete medium containing 10% CCK-8 reagent for 2 hours.
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