Author: Shefali Dobhal; Gamze Boluk; Brooke Babler; Michael J. Stulberg; John Rascoe; Mark Nakhla; Toni A. Chapman; Alex B. Crockford; Michael Melzer; Anne M. Alvarez; Mohammad Arif
Title: Comparative genomics reveals signature regions used to develop a robust and sensitive multiplex TaqMan real-time qPCR assay to detect the genus Dickeya and Dickeya dianthicola Document date: 2019_11_20
ID: lgeu4id0_28
Snippet: No change in the sensitivity was observed when 1 µl of host genomic DNA (DNA extracted from healthy potato plant) was added to each ten-fold serially diluted genomic DNA. The detection limit of 10 fg was detected; demonstrating the robustness of the developed assay with no adverse effect from host background DNA ( Figure 5C1, C2) . The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10......
Document: No change in the sensitivity was observed when 1 µl of host genomic DNA (DNA extracted from healthy potato plant) was added to each ten-fold serially diluted genomic DNA. The detection limit of 10 fg was detected; demonstrating the robustness of the developed assay with no adverse effect from host background DNA ( Figure 5C1, C2) . The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/847590 doi: bioRxiv preprint
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