Author: Zheng, Li-Zhen; Wang, Jia-Li; Kong, Ling; Huang, Le; Tian, Li; Pang, Qian-Qian; Wang, Xin-Luan; Qin, Ling
Title: Steroid-associated osteonecrosis animal model in rats Document date: 2018_2_6
ID: tad9jmfp_14
Snippet: Perfusion and decalcification: Under general and deep anaesthesia with intravenous administration of 2.5% sodium pentobarbital, the abdomen cavity of the rats were opened for perfusion with a confected radiopaque silicone rubber (Microfil MV-122; Flow Tech, Inc., Carver, MA, USA) via the abdominal aorta using our previously established protocol (n Z 4/group) [3, 20, 21] . The animals were then sacrificed and stored at 4 C for full polymerisation .....
Document: Perfusion and decalcification: Under general and deep anaesthesia with intravenous administration of 2.5% sodium pentobarbital, the abdomen cavity of the rats were opened for perfusion with a confected radiopaque silicone rubber (Microfil MV-122; Flow Tech, Inc., Carver, MA, USA) via the abdominal aorta using our previously established protocol (n Z 4/group) [3, 20, 21] . The animals were then sacrificed and stored at 4 C for full polymerisation of the in vivo injected Microfil. The excised tibiae were decalcified by 10% EDTA (pH 7.4). The decalcification process was assessed radiographically to confirm complete decalcification, i.e., without radiographic opacity.
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