Selected article for: "cytoplasmic domain and immunofluorescence microscopy"

Title: trans-Golgi retention of a plasma membrane protein: mutations in the cytoplasmic domain of the asialoglycoprotein receptor subunit H1 result in trans-Golgi retention
  • Document date: 1995_7_2
  • ID: tedj3xxz_38
    Snippet: To define the determinants for trans-Golgi accumulation, we prepared a series of H1 cDNA constructs with alterations in the cytoplasmic domain, shown in Fig. 1 . These constructs were transiently expressed in MDCK cells and analyzed by indirect immunofluorescence microscopy for accumulation in the typical juxtanuclear, tubular Golgi structures vs transport to the plasma membrane. The results are summarized in Fig. 1 . The immunofluorescence patte.....
    Document: To define the determinants for trans-Golgi accumulation, we prepared a series of H1 cDNA constructs with alterations in the cytoplasmic domain, shown in Fig. 1 . These constructs were transiently expressed in MDCK cells and analyzed by indirect immunofluorescence microscopy for accumulation in the typical juxtanuclear, tubular Golgi structures vs transport to the plasma membrane. The results are summarized in Fig. 1 . The immunofluorescence patterns of the most relevant constructs are shown in Fig. 8 (A-C) .

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