Antimicrobial activity of novel synthesized coumarin based transitional metal complexes

Sahoo, Jyotirmaya; Paidesetty, Sudhir K.

The different concentrations for the respective compounds were loaded into the wells and incubated at 37 C for 18e 24 h. After incubation, the MIC was determined. 9 Acute toxicity study The said synthesized compounds were subjected to an acute oral toxicity study to establish their safety dose. Healthy young female Wister rats that were 8e12 weeks of age were selected. OECD guideline No. 420 (2000) for the acute oral toxicity fixed dose procedure of the test compounds (3ae3b and 4ae4h) was followed. To conduct the sighting study, a single animal was provided with suspensions of the synthesized compounds having a specific dose, viz. 5 mg, 50 mg, 300 mg and 2000 mg/kg body weight, with the aid of an intubation canula. A period of 24 h was maintained between each dosing. In the main study, another 4 animals were administered 2000 mg/kg. The animals were observed for a period of 14 days. However, the acute toxic symptoms and the behavioural changes produced by the test compounds were observed continuously at an interval of 4 h up to 24 h.