Author: Stewart, Meredith E.; Roy, Polly
Title: Structure-based identification of functional residues in the nucleoside-2'-O-methylase domain of Bluetongue virus VP4 capping enzyme Document date: 2015_2_24
ID: vzel6r43_16
Snippet: The dsRNA genome was extracted from virus infected cells as previously described [32] and analyzed on TBE agarose gel. Purified dsRNAs were used as templates to reverse transcribe S4 to generate cDNA using SuperScript III (Invitrogen). S4 was amplified using specific primers, using KOD polymerase. PCR products were sequenced to ensure that the introduced mutations were stable and that there were no compensatory changes to the S4......
Document: The dsRNA genome was extracted from virus infected cells as previously described [32] and analyzed on TBE agarose gel. Purified dsRNAs were used as templates to reverse transcribe S4 to generate cDNA using SuperScript III (Invitrogen). S4 was amplified using specific primers, using KOD polymerase. PCR products were sequenced to ensure that the introduced mutations were stable and that there were no compensatory changes to the S4.
Search related documents:
Co phrase search for related documents- agarose gel and PCR product: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- agarose gel and specific primer: 1, 2, 3, 4, 5, 6
- agarose gel and SuperScript III Invitrogen: 1
- agarose gel and TBE agarose gel: 1, 2, 3
- cdna generate and PCR product: 1
- dsrna genome and infect cell: 1
- infect cell and virus infect cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- introduce mutation and PCR product: 1
- KOD polymerase and SuperScript III Invitrogen: 1
- PCR product and specific primer: 1, 2, 3, 4, 5, 6
- PCR product and SuperScript III Invitrogen: 1
- previously describe and SuperScript III Invitrogen: 1
- specific primer and SuperScript III Invitrogen: 1, 2, 3
Co phrase search for related documents, hyperlinks ordered by date