Selected article for: "copy number and PCR product"
Author: Yu Jin Jung; Gun-Soo Park; Jun Hye Moon; Keunbon Ku; Seung-Hwa Beak; Seil Kim; Edmond Changkyun Park; Daeui Park; Jong-Hwan Lee; Cheol Woo Byeon; Joong Jin Lee; Jin-Soo Maeng; Seong Jun Kim; Seung Il Kim; Bum-Tae Kim; Min Jun Lee; Hong Gi Kim
Title: Comparative analysis of primer-probe sets for the laboratory confirmation of SARS-CoV-2
  • Document date: 2020_2_27
    • ID: 2hyiped2_10
    Snippet: The coding sequence of SARS-CoV-2 Envelope (E) protein, which cloned in pET21a plasmid was PCR amplified with T7 promoter primer (5' -AATACGACTCACTATAG -3', Macrogen Inc., South Korea) and T7 terminator primer (5' -GCTAGTTATTGCTCAGCGG -3', Macrogen) with AccuPower® PCR PreMix (-dye) kit (Bioneer Inc., South Korea). PCR product was then used as in vitro transcription template using MEGAscript™ T7 Transcription Kit (Invitrogen Inc., CA, USA). Th.....
    Document: The coding sequence of SARS-CoV-2 Envelope (E) protein, which cloned in pET21a plasmid was PCR amplified with T7 promoter primer (5' -AATACGACTCACTATAG -3', Macrogen Inc., South Korea) and T7 terminator primer (5' -GCTAGTTATTGCTCAGCGG -3', Macrogen) with AccuPower® PCR PreMix (-dye) kit (Bioneer Inc., South Korea). PCR product was then used as in vitro transcription template using MEGAscript™ T7 Transcription Kit (Invitrogen Inc., CA, USA). The copy number of in vitro transcribed RNA was calculated from . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.02.25.964775 doi: bioRxiv preprint RNA concentration measured with Quantus™ Fluorometer (Promega Inc., WI, USA). Standardized amounts of in vitro produced RNA were used E primer and qRT-PCR to produce a standard curve.

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