Author: Teoh, Kim-Tat; Siu, Yu-Lam; Chan, Wing-Lim; Schlüter, Marc A.; Liu, Chia-Jen; Peiris, J. S. Malik; Bruzzone, Roberto; Margolis, Benjamin; Nal, Béatrice
Title: The SARS Coronavirus E Protein Interacts with PALS1 and Alters Tight Junction Formation and Epithelial Morphogenesis Document date: 2010_11_15
ID: ufw13pjx_13
Snippet: For production of the MDCKII stable cell line expressing eGFP-PALS1, the PALS1 cDNA was introduced into the EGFP fusion retroviral vector pLEGFP-C1 (BD Biosciences, San Jose, CA) as previously described (Kamberov et al., 2000). For production of MDCKII stable cell lines expressing the HA-E (wt) and (ΔPBM), retroviral constructs were generated by inserting full length (wt) or truncated (ΔPBM) E nucleotide sequences, excised from pcDNA by digesti.....
Document: For production of the MDCKII stable cell line expressing eGFP-PALS1, the PALS1 cDNA was introduced into the EGFP fusion retroviral vector pLEGFP-C1 (BD Biosciences, San Jose, CA) as previously described (Kamberov et al., 2000). For production of MDCKII stable cell lines expressing the HA-E (wt) and (ΔPBM), retroviral constructs were generated by inserting full length (wt) or truncated (ΔPBM) E nucleotide sequences, excised from pcDNA by digestion with BamHI and XhoI, into the pCHMWS-eGFP-Hygro retroviral vector (kindly provided by Dr. Rik Gijsbers, Molecular Medicine at the Katholieke Universiteit Leuven, Belgium) after removal of the EGFP gene.
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