Author: Teoh, Kim-Tat; Siu, Yu-Lam; Chan, Wing-Lim; Schlüter, Marc A.; Liu, Chia-Jen; Peiris, J. S. Malik; Bruzzone, Roberto; Margolis, Benjamin; Nal, Béatrice
Title: The SARS Coronavirus E Protein Interacts with PALS1 and Alters Tight Junction Formation and Epithelial Morphogenesis Document date: 2010_11_15
ID: ufw13pjx_16
Snippet: The rabbit polyclonal antibody against the carboxy-terminal domain of the E protein was previously described (Siu et al., 2008). The mouse polyclonal antibody against the SARS-S was raised by immunizing mice with purified S trimers expressed in mammalian cells as described (Kam et al., 2007). The mouse monoclonal anti-ERGIC 53 (Schweizer et al., 1988) was a generous gift from Hans-Peter Hauri (Biozentrum, University Basel). The rabbit polyclonal .....
Document: The rabbit polyclonal antibody against the carboxy-terminal domain of the E protein was previously described (Siu et al., 2008). The mouse polyclonal antibody against the SARS-S was raised by immunizing mice with purified S trimers expressed in mammalian cells as described (Kam et al., 2007). The mouse monoclonal anti-ERGIC 53 (Schweizer et al., 1988) was a generous gift from Hans-Peter Hauri (Biozentrum, University Basel). The rabbit polyclonal PALS1 and CRB3 antisera were produced as described (Kamberov et al., 2000; Makarova et al., 2003). The antibodies anti-Calnexin and Golgin-97 were mouse monoclonal antibodies from Abnova (Taipei City, Taiwan) and Invitrogen, respectively. The rabbit polyclonal antibody anti-Giantin was from Covance (Princeton, NJ). George Ojakian at the SUNY Downstate Medical Center (Brooklyn, NY) kindly provided the mouse antibody against GP135. A rat IgG1 monoclonal anti-HA (clone 3F10, Roche Diagnostic GmbH) was used for immunofluorescence microscopy to detect HA-E (wt) and HA-E (ΔPBM) in MDCKII cell lines. The mouse and rat IgG1 monoclonal antibodies against zonula occludens (ZO-1) and E-cadherin proteins were purchased from Invitrogen. For immunofluorescence microscopy, goat anti-rabbit FITC, donkey anti-mouse Texas Red (Jackson ImmunoResearch Laboratories, West Grove, PA), goat anti-mouse FITC (ZyMax, Invitrogen), goat anti-mouse Texas Red (Oncogene-Calbiochem, Gibbstown, NJ), Alexa Fluor-647 goat anti-mouse and goat anti-rat, Alexa Fluor-555 goat anti-rat and goat anti-mouse, and Alexa Fluor®-488 goat anti-rabbit and goat anti-mouse were used as fluorochrome-conjugated secondary antibodies (Invitrogen). For immunoblotting, goat anti-rabbit, anti-rat, and anti-mouse horse-radish peroxidase (HRP)-conjugated secondary antibodies were used (ZyMed, Invitrogen).
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