Author: Josset, Laurence; Zeng, Hui; Kelly, Sara M.; Tumpey, Terrence M.; Katze, Michael G.
Title: Transcriptomic Characterization of the Novel Avian-Origin Influenza A (H7N9) Virus: Specific Host Response and Responses Intermediate between Avian (H5N1 and H7N7) and Human (H3N2) Viruses and Implications for Treatment Options Document date: 2014_2_4
ID: uz0m1o0q_25
Snippet: Cell culture and viral infection. The human bronchial epithelial cell line Calu-3 was originally derived from subbronchial epithelium of a lung adenocarcinoma (ATCC), and cells were grown as previously described (19) . Confluent monolayers of polarized Calu-3 cells achieving stable transepithelial resistance were infected apically at an MOI of 1. After a 1-h incubation, monolayers were washed to remove nonadherent virus and 2 ml of minimal essent.....
Document: Cell culture and viral infection. The human bronchial epithelial cell line Calu-3 was originally derived from subbronchial epithelium of a lung adenocarcinoma (ATCC), and cells were grown as previously described (19) . Confluent monolayers of polarized Calu-3 cells achieving stable transepithelial resistance were infected apically at an MOI of 1. After a 1-h incubation, monolayers were washed to remove nonadherent virus and 2 ml of minimal essential medium-bovine serum albumin (MEM-BSA) was added to both apical and basolateral reservoirs of cells and left for the duration of the experiment.
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