Title: Susceptibility to cytotoxic T lymphocyte-induced apoptosis is a function of the proliferative status of the target Document date: 1994_2_1
ID: s0bfhwtn_12
Snippet: Figttre 4. Target call DNA is fragmented into the nucleosomal ladder pattern typical of apoptosis. Antoradiogram of target cell DNA purified as described from either the assay medium (214) or from the cell pellet (P) after the medium had been removed or from target ceils, only, without CTL added (C) (5) . Targets were exposed to CTL for either 6 (P815, A20) or 10 h (b929, 3T3-A31, A31-ngr ) assays. DNA fragmentation was assessed from samples at a.....
Document: Figttre 4. Target call DNA is fragmented into the nucleosomal ladder pattern typical of apoptosis. Antoradiogram of target cell DNA purified as described from either the assay medium (214) or from the cell pellet (P) after the medium had been removed or from target ceils, only, without CTL added (C) (5) . Targets were exposed to CTL for either 6 (P815, A20) or 10 h (b929, 3T3-A31, A31-ngr ) assays. DNA fragmentation was assessed from samples at an E/T ratio of 50:1. Control samples were from targets without director cells. 30,000 cpm + 10% of each sample component was loaded onto 1.5% agarose gels. Gels were dried and exposed at -80oc with two screens (5).
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