Author: Menachery, Vineet D.; Eisfeld, Amie J.; Schäfer, Alexandra; Josset, Laurence; Sims, Amy C.; Proll, Sean; Fan, Shufang; Li, Chengjun; Neumann, Gabriele; Tilton, Susan C.; Chang, Jean; Gralinski, Lisa E.; Long, Casey; Green, Richard; Williams, Christopher M.; Weiss, Jeffrey; Matzke, Melissa M.; Webb-Robertson, Bobbie-Jo; Schepmoes, Athena A.; Shukla, Anil K.; Metz, Thomas O.; Smith, Richard D.; Waters, Katrina M.; Katze, Michael G.; Kawaoka, Yoshihiro; Baric, Ralph S.
Title: Pathogenic Influenza Viruses and Coronaviruses Utilize Similar and Contrasting Approaches To Control Interferon-Stimulated Gene Responses Document date: 2014_5_20
ID: s3zeppze_30
Snippet: RNA isolation, microarray processing, and identification of differential expression. RNA isolation and microarray processing from Calu3 cells were carried out as previously described (5) . Differential expression (DE) was determined by comparing virus-infected replicates to timematched mock replicates. Criteria for DE in determining the consensus ISG list were an absolute log 2 FC of Ͼ1.5 and a false discovery rate (FDR)adjusted P value of Ͻ0.0.....
Document: RNA isolation, microarray processing, and identification of differential expression. RNA isolation and microarray processing from Calu3 cells were carried out as previously described (5) . Differential expression (DE) was determined by comparing virus-infected replicates to timematched mock replicates. Criteria for DE in determining the consensus ISG list were an absolute log 2 FC of Ͼ1.5 and a false discovery rate (FDR)adjusted P value of Ͻ0.05 for a given time point.
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