Selected article for: "confocal fluorescence microscope and fluorescence microscope"
Author: Vandergriff, Adam; Huang, Ke; Shen, Deliang; Hu, Shiqi; Hensley, Michael Taylor; Caranasos, Thomas G.; Qian, Li; Cheng, Ke
Title: Targeting regenerative exosomes to myocardial infarction using cardiac homing peptide
  • Document date: 2018_2_14
    • ID: yi8zv3co_22
    Snippet: All rat specimens were sacrificed at day 21. For immunohistochemistry (IHC), heart sections were then fixed in 4% paraformaldehyde solution, permeabilized with 0.01% saponin (Sigma-Aldrich, St. Louis, MO), and blocked using Protein Block solution (Dako; Glostrup, Denmark). Primary antibodies were incubated overnight at 4 °C, and subsequently secondary antibodies at room temperature for 1.5 h. Samples were then treated with DAPI (LifeTech, Carlsb.....
    Document: All rat specimens were sacrificed at day 21. For immunohistochemistry (IHC), heart sections were then fixed in 4% paraformaldehyde solution, permeabilized with 0.01% saponin (Sigma-Aldrich, St. Louis, MO), and blocked using Protein Block solution (Dako; Glostrup, Denmark). Primary antibodies were incubated overnight at 4 °C, and subsequently secondary antibodies at room temperature for 1.5 h. Samples were then treated with DAPI (LifeTech, Carlsbad, CA) and mounted in Prolong Gold Mounting Media (LifeTech, Carlsbad, CA). Images were acquired using an Olympus IX81 fluorescence microscope and Zeiss LSM710 confocal microscope. Image processing and analysis was performed using ImageJ. For analyzing DiI uptake, nuclear staining was subtracted prior to measurement to avoid detection of Ki67 labelling.

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