Selected article for: "control cell and Golgi region"
Author: Teoh, Kim-Tat; Siu, Yu-Lam; Chan, Wing-Lim; Schlüter, Marc A.; Liu, Chia-Jen; Peiris, J. S. Malik; Bruzzone, Roberto; Margolis, Benjamin; Nal, Béatrice
Title: The SARS Coronavirus E Protein Interacts with PALS1 and Alters Tight Junction Formation and Epithelial Morphogenesis
  • Document date: 2010_11_15
    • ID: ufw13pjx_64
    Snippet: Strikingly, we observed that ectopic expression of HA-E protein in MDCKII eGFP-PALS1 cells was associated with formation of cysts with either two or multiple lumens. HA-E protein was distributed in the subapical region of the cysts, as shown by its localization underneath the GP135 apical protein, which is consistent with localization in the ERGIC/Golgi region (Figure 5B). Quantitative analysis showed that only 21% (187/874) of cysts expressing H.....
    Document: Strikingly, we observed that ectopic expression of HA-E protein in MDCKII eGFP-PALS1 cells was associated with formation of cysts with either two or multiple lumens. HA-E protein was distributed in the subapical region of the cysts, as shown by its localization underneath the GP135 apical protein, which is consistent with localization in the ERGIC/Golgi region (Figure 5B). Quantitative analysis showed that only 21% (187/874) of cysts expressing HA-E (wt) proteins had formed correctly (Figure 5C), a threefold decrease compared with control cell lines (p < 0.001, unpaired t test).

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