Author: Teoh, Kim-Tat; Siu, Yu-Lam; Chan, Wing-Lim; Schlüter, Marc A.; Liu, Chia-Jen; Peiris, J. S. Malik; Bruzzone, Roberto; Margolis, Benjamin; Nal, Béatrice
Title: The SARS Coronavirus E Protein Interacts with PALS1 and Alters Tight Junction Formation and Epithelial Morphogenesis Document date: 2010_11_15
ID: ufw13pjx_8
Snippet: Madin-Darby Canine Kidney II (MDCKII) epithelial cells were previously characterized (Gaush et al., 1966). Vero E6 African Green Monkey kidney (ATCC, Manassas, VA, USA), Human Embryonic Kidney (HEK) 293T (ATCC), and MDCKII epithelial cell lines were cultured in Dulbecco's Modified Eagle's medium (DMEM) (Invitrogen, Carlsbad, CA, USA) supplemented with 10% heat-inactivated fetal calf serum (FCS), 100 U/ml penicillin and 100 μg/ml streptomycin (In.....
Document: Madin-Darby Canine Kidney II (MDCKII) epithelial cells were previously characterized (Gaush et al., 1966). Vero E6 African Green Monkey kidney (ATCC, Manassas, VA, USA), Human Embryonic Kidney (HEK) 293T (ATCC), and MDCKII epithelial cell lines were cultured in Dulbecco's Modified Eagle's medium (DMEM) (Invitrogen, Carlsbad, CA, USA) supplemented with 10% heat-inactivated fetal calf serum (FCS), 100 U/ml penicillin and 100 μg/ml streptomycin (Invitrogen). Cells were incubated at 37°C in a 95% O2/5% CO2 atmosphere. Well-differentiated normal human bronchial epithelial (wd-NHBE) cells were cultured as described previously (Chan et al., 2010).
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