Author: Li, Dongbo; Ji, Hongfei; Niu, Xingjian; Yin, Lei; Wang, Yiran; Gu, Yucui; Wang, Jinlu; Zhou, Xiaoping; Zhang, Han; Zhang, Qingyuan
Title: Tumor-associated macrophages secrete CC-chemokine ligand 2 and induce tamoxifen resistance by activating PI3K/Akt/mTOR in breast cancer Document date: 2019_12_19
ID: yj9mb88g_24
Snippet: Survival curves were analyzed using Kaplan-Meier method and curves were compared using the log-rank test. Multivariate survival analysis using the Cox regression proportional hazards model was carried out to adjust for clinical variables that may have statistical significance for prognosis in a univariate analysis. Results with P values <.05 were considered to be statistically significant (*P < .05, **P < .01). To examine the differences in the m.....
Document: Survival curves were analyzed using Kaplan-Meier method and curves were compared using the log-rank test. Multivariate survival analysis using the Cox regression proportional hazards model was carried out to adjust for clinical variables that may have statistical significance for prognosis in a univariate analysis. Results with P values <.05 were considered to be statistically significant (*P < .05, **P < .01). To examine the differences in the macrophages, cellular CD163 immunofluorescence and flow cytometry analysis were carried out on the MΦ, MS, and MR. CD163 is considered a polarization maker for TAM and M2. We found that CM from breast cancer cell lines increased CD163 expression in MΦ, indicating M2 polarization.
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