Selected article for: "lysis buffer and passive lysis buffer"
Author: Ishibashi, Daisuke; Homma, Takujiro; Nakagaki, Takehiro; Fuse, Takayuki; Sano, Kazunori; Satoh, Katsuya; Mori, Tsuyoshi; Atarashi, Ryuichiro; Nishida, Noriyuki
Title: Type I interferon protects neurons from prions in in vivo models
  • Document date: 2019_2_7
    • ID: zopwlaq4_16
    Snippet: Ifnar1 À/À and Ifnar1 gene-transduced Ifnar1 À/À (Ifnar1-Ifnar1 À/À ) MEF cells were transiently co-transfected with the plasmids pUNO-ISRE-luc and pRL-null as internal standard plasmids, using Lipofectamine TM LTX, and cultured for 24 h. The treated cells were continuously transfected with 30 mg/ml Poly I:C using Lipofectamine TM LTX. After 20 h, the cells were lysed using Passive Lysis Buffer (Promega) and the luciferase activity was dete.....
    Document: Ifnar1 À/À and Ifnar1 gene-transduced Ifnar1 À/À (Ifnar1-Ifnar1 À/À ) MEF cells were transiently co-transfected with the plasmids pUNO-ISRE-luc and pRL-null as internal standard plasmids, using Lipofectamine TM LTX, and cultured for 24 h. The treated cells were continuously transfected with 30 mg/ml Poly I:C using Lipofectamine TM LTX. After 20 h, the cells were lysed using Passive Lysis Buffer (Promega) and the luciferase activity was detected by the Dual Reporter Assay System (Promega) and quantified using the Mithras LB940 luminometer instrument (Berthold Technologies). Data of luciferase activity were normalized by value of the co-expressed Renilla activity as previously described (Homma et al., 2014a) .

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