Selected article for: "cdna quantitative PCR and quantitative PCR"
Author: Galindo, I; Hernáez, B; Muñoz-Moreno, R; Cuesta-Geijo, M A; Dalmau-Mena, I; Alonso, C
Title: The ATF6 branch of unfolded protein response and apoptosis are activated to promote African swine fever virus infection
  • Document date: 2012_7_5
    • ID: qfm61wmx_24
    Snippet: For quantitative PCR, cDNA was synthesized using the QuantiTect Reverse Trancription kit (Qiagen, Hilden, Germany) following the manufacturer's instructions. After heat inactivation at 95 1C, cDNA levels were quantified by using quantitative PCR (qPCR). Amplification was performed in a Rotor-Gene 3000 (Corbett Life Sciences, Sydney, Australia) using a QuantiTect SYBR Green PCR kit (Qiagen) following the manufacturer's recommendations. Primer sets.....
    Document: For quantitative PCR, cDNA was synthesized using the QuantiTect Reverse Trancription kit (Qiagen, Hilden, Germany) following the manufacturer's instructions. After heat inactivation at 95 1C, cDNA levels were quantified by using quantitative PCR (qPCR). Amplification was performed in a Rotor-Gene 3000 (Corbett Life Sciences, Sydney, Australia) using a QuantiTect SYBR Green PCR kit (Qiagen) following the manufacturer's recommendations. Primer sets used for 18S and XBP1 are available from Qiagen (QuantiTect Primer Assay). Fold change was calculated using the DDCT method of relative quantification with 18S rRNA as the endogenous control for normalization. 43 Melting curves were used to verify the specificities of products.

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