Selected article for: "binding model and curve fitting"
Author: Klaile, Esther; Vorontsova, Olga; Sigmundsson, Kristmundur; Müller, Mario M.; Singer, Bernhard B.; Öfverstedt, Lars-Göran; Svensson, Stina; Skoglund, Ulf; Öbrink, Björn
Title: The CEACAM1 N-terminal Ig domain mediates cis- and trans-binding and is essential for allosteric rearrangements of CEACAM1 microclusters
  • Document date: 2009_11_16
    • ID: uy2553z7_10
    Snippet: In the presence of EDTA, all three models gave equally good curve fitting ( Fig. 1 A) with no significant differences in the  2 values calculated for all data points in the association and dissociation phases. Model 2 was the least plausible because it gave unacceptable large standard deviations for the rate constants. Analysis according to model 3 gave acceptable standard deviations but indicated that the formation of type 2 dimers was almost.....
    Document: In the presence of EDTA, all three models gave equally good curve fitting ( Fig. 1 A) with no significant differences in the  2 values calculated for all data points in the association and dissociation phases. Model 2 was the least plausible because it gave unacceptable large standard deviations for the rate constants. Analysis according to model 3 gave acceptable standard deviations but indicated that the formation of type 2 dimers was almost insignificant. Thus, we conclude that in the presence of EDTA, essentially only the formation of type 1 dimers was recorded. Analysis according to model 1 gave the following values of the binding constants in EDTA: k a1 = 0.0453 ± 0.0036 µM 1 s 1 ; k d1 = 0.5083 ± 0.0078 s 1 ; and K D1 = 11.21 µM.

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