Author: te Velthuis, Aartjan J.W.; van den Worm, Sjoerd H. E.; Snijder, Eric J.
Title: The SARS-coronavirus nsp7+nsp8 complex is a unique multimeric RNA polymerase capable of both de novo initiation and primer extension Document date: 2011_10_29
ID: tx0lqgff_24
Snippet: To investigate whether nsp7 could influence the change in multimerisation behaviour, we next added separately purified and C-terminally processed nsp7 to the different nsp8 preparations. Interestingly, we found that nsp8 and nsp8-His could both associate with this protein, in accordance with published data (13) , but that His-nsp8 was unable to do so within the frame of our experimental conditions ( Figure 2F ). Consequently, although various lin.....
Document: To investigate whether nsp7 could influence the change in multimerisation behaviour, we next added separately purified and C-terminally processed nsp7 to the different nsp8 preparations. Interestingly, we found that nsp8 and nsp8-His could both associate with this protein, in accordance with published data (13) , but that His-nsp8 was unable to do so within the frame of our experimental conditions ( Figure 2F ). Consequently, although various lines of evidence support the observation that nsp7 and nsp8 can form a hexadecamer, it now appears that the correct N-terminal processing of nsp8 is a significant factor in determining the final oligomeric state of the protein.
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