Selected article for: "frame reading and insert reading frame orientation"

Title: The single transmembrane segment of gp210 is sufficient for sorting to the pore membrane domain of the nuclear envelope
  • Document date: 1992_12_2
  • ID: vznqgnzd_12
    Snippet: This oligonucleotide pair was inserted into a HindIII site (nucleotide 79) of the gp210 eDNA. The orientation of the insert and the reading frame of the resulting gp210:HA eDNA was confirmed by DNA sequencing. The gp210:HA eDNA was subcloned into the XhoI site of the eukaryotic expression vector pSVL (Pharmacia LKB Biotechnology, Piscataway, NJ)......
    Document: This oligonucleotide pair was inserted into a HindIII site (nucleotide 79) of the gp210 eDNA. The orientation of the insert and the reading frame of the resulting gp210:HA eDNA was confirmed by DNA sequencing. The gp210:HA eDNA was subcloned into the XhoI site of the eukaryotic expression vector pSVL (Pharmacia LKB Biotechnology, Piscataway, NJ).

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