Author: Klaile, Esther; Vorontsova, Olga; Sigmundsson, Kristmundur; Müller, Mario M.; Singer, Bernhard B.; Öfverstedt, Lars-Göran; Svensson, Stina; Skoglund, Ulf; Öbrink, Björn
Title: The CEACAM1 N-terminal Ig domain mediates cis- and trans-binding and is essential for allosteric rearrangements of CEACAM1 microclusters Document date: 2009_11_16
ID: uy2553z7_56
Snippet: 3 ] form, the curve-fit model is composed of the following 10 coupled differential equations, applied for numerical integration: were analyzed by SDS-PAGE under reducing conditions, and Western blotting was performed with rat CEACAM1-specific Mab Be9.2 (Becker et al., 1986) or Pab î¡CC16 (Singer et al., 2000) to detect D(1-4)-His and D(2-4)-His, respectively. Protein detection on Western blots using nitrocellulose membrane (Schleicher & Schüll).....
Document: 3 ] form, the curve-fit model is composed of the following 10 coupled differential equations, applied for numerical integration: were analyzed by SDS-PAGE under reducing conditions, and Western blotting was performed with rat CEACAM1-specific Mab Be9.2 (Becker et al., 1986) or Pab î¡CC16 (Singer et al., 2000) to detect D(1-4)-His and D(2-4)-His, respectively. Protein detection on Western blots using nitrocellulose membrane (Schleicher & Schüll) was performed using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific). Chemiluminescence was detected using a digital system (LAS-1000; Fujifilm). Quantification was performed using Image Gauge software (Fujifilm). Images were imported into Photoshop (Adobe) for processing.
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