Author: Kopertekh, Lilya; Meyer, Torsten; Freyer, Cornelia; Hust, Michael
Title: Transient plant production of Salmonella Typhimurium diagnostic antibodies Document date: 2019_2_12
ID: y47ahl3p_2
Snippet: For a sensitive and specific ELISA, new immunogenic and speciesspecific proteins are required. One of the major proteins of the S. Typhimurium outer membrane, the 39 kDa OmpD protein, is a promising candidates to develop corresponding diagnostic antibodies. It is expressed in addition to OmpF and OmpC proteins and is shown to be immunogenic [8, 9] . Recently, human recombinant antibody fragments (scFv) were isolated from the naive human antibody .....
Document: For a sensitive and specific ELISA, new immunogenic and speciesspecific proteins are required. One of the major proteins of the S. Typhimurium outer membrane, the 39 kDa OmpD protein, is a promising candidates to develop corresponding diagnostic antibodies. It is expressed in addition to OmpF and OmpC proteins and is shown to be immunogenic [8, 9] . Recently, human recombinant antibody fragments (scFv) were isolated from the naive human antibody gene library HAL7/8 by phage display using the OmpD protein as an antigen [10] . The scFv-TM43-E10 antibody was further characterized with the aim to develop diagnostic assay [11] .
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