Author: Kopertekh, Lilya; Meyer, Torsten; Freyer, Cornelia; Hust, Michael
Title: Transient plant production of Salmonella Typhimurium diagnostic antibodies Document date: 2019_2_12
ID: y47ahl3p_35_1
Snippet: s study we demonstrate for the first time the effectiveness of gb to increase the recombinant protein expression level. The increased transcription of scFv-TM43-E10 and scFv-Fc-TM43-E10 genes was detected by qPCR analysis in N. benthamiana leaves agroinfiltrated with pLHgb-PVX-m vector when compared to pLH-PVX-m expression vector. The mRNA expression levels correlated with protein yields as was quantified by ELISA. Application of the gb silencing.....
Document: s study we demonstrate for the first time the effectiveness of gb to increase the recombinant protein expression level. The increased transcription of scFv-TM43-E10 and scFv-Fc-TM43-E10 genes was detected by qPCR analysis in N. benthamiana leaves agroinfiltrated with pLHgb-PVX-m vector when compared to pLH-PVX-m expression vector. The mRNA expression levels correlated with protein yields as was quantified by ELISA. Application of the gb silencing suppressor caused two and five fold increases in scFv-TM43-E10 and scFv-Fc-TM43-E10 accumulation, respectively.
Search related documents:
Co phrase search for related documents- expression level and increased transcription: 1, 2, 3, 4
- expression level and mrna expression level: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74
- expression level and protein expression: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81
- expression level and protein expression level: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78
- expression level and protein yield: 1, 2, 3, 4, 5, 6, 7, 8
- expression level and recombinant protein expression level: 1, 2, 3, 4, 5, 6, 7
- expression level and scFv Fc accumulation: 1, 2
- expression level and scFv Fc scFv: 1, 2, 3, 4
- expression level and scfv scFv Fc accumulation: 1, 2
- expression vector and fold increase: 1, 2, 3
- expression vector and protein expression: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76
- expression vector and protein expression level: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- expression vector and protein yield: 1, 2, 3, 4, 5, 6, 7
- expression vector and recombinant protein expression level: 1, 2, 3
- expression vector and scFv Fc accumulation: 1, 2, 3
- expression vector and scFv Fc scFv: 1, 2, 3, 4
- expression vector and scfv scFv Fc accumulation: 1, 2, 3
- fold increase and increased transcription: 1, 2, 3, 4, 5
- fold increase and mrna expression level: 1
Co phrase search for related documents, hyperlinks ordered by date