Selected article for: "expression level and mRNA expression"
Author: Kopertekh, Lilya; Meyer, Torsten; Freyer, Cornelia; Hust, Michael
Title: Transient plant production of Salmonella Typhimurium diagnostic antibodies
  • Document date: 2019_2_12
    • ID: y47ahl3p_35_1
    Snippet: s study we demonstrate for the first time the effectiveness of gb to increase the recombinant protein expression level. The increased transcription of scFv-TM43-E10 and scFv-Fc-TM43-E10 genes was detected by qPCR analysis in N. benthamiana leaves agroinfiltrated with pLHgb-PVX-m vector when compared to pLH-PVX-m expression vector. The mRNA expression levels correlated with protein yields as was quantified by ELISA. Application of the gb silencing.....
    Document: s study we demonstrate for the first time the effectiveness of gb to increase the recombinant protein expression level. The increased transcription of scFv-TM43-E10 and scFv-Fc-TM43-E10 genes was detected by qPCR analysis in N. benthamiana leaves agroinfiltrated with pLHgb-PVX-m vector when compared to pLH-PVX-m expression vector. The mRNA expression levels correlated with protein yields as was quantified by ELISA. Application of the gb silencing suppressor caused two and five fold increases in scFv-TM43-E10 and scFv-Fc-TM43-E10 accumulation, respectively.

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