Selected article for: "flow cytometry and plasma membrane"
Author: Schrom, Eva; Huber, Maja; Aneja, Manish; Dohmen, Christian; Emrich, Daniela; Geiger, Johannes; Hasenpusch, Günther; Herrmann-Janson, Annika; Kretzschmann, Verena; Mykhailyk, Olga; Pasewald, Tamara; Oak, Prajakta; Hilgendorff, Anne; Wohlleber, Dirk; Hoymann, Heinz-Gerd; Schaudien, Dirk; Plank, Christian; Rudolph, Carsten; Kubisch-Dohmen, Rebekka
Title: Translation of Angiotensin-Converting Enzyme 2 upon Liver- and Lung-Targeted Delivery of Optimized Chemically Modified mRNA
  • Document date: 2017_4_13
    • ID: tulmnb32_38
    Snippet: Correct protein integration into the plasma membrane was assessed by flow cytometry. Cells were washed with PBS, detached with TrypLE (Gibco/Life Technologies), and re-suspended in flow cytometry buffer (PBS supplemented with 10% FCS). Cells were incubated with primary antibody against ACE2 (5 mg/mL, AF933; R&D Systems) in flow cytometry buffer for 1 hr at 4 C. After washing with flow cytometry buffer, anti-goat AF488 antibody (1:400, A11087; The.....
    Document: Correct protein integration into the plasma membrane was assessed by flow cytometry. Cells were washed with PBS, detached with TrypLE (Gibco/Life Technologies), and re-suspended in flow cytometry buffer (PBS supplemented with 10% FCS). Cells were incubated with primary antibody against ACE2 (5 mg/mL, AF933; R&D Systems) in flow cytometry buffer for 1 hr at 4 C. After washing with flow cytometry buffer, anti-goat AF488 antibody (1:400, A11087; Thermo Fisher Scientific) was added for 1 hr at 4 C. Cells were washed again in flow cytometry buffer and stained with propidium iodide for discrimination between live and dead cells (1 mg/mL Sigma-Aldrich). Analysis was performed on an Attune Acoustic Focusing Cytometer (Life Technologies) with Attune Cytometric Software (version 2.1; Life Technologies) and FlowJo (version 10).

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