Author: Li, Dongbo; Ji, Hongfei; Niu, Xingjian; Yin, Lei; Wang, Yiran; Gu, Yucui; Wang, Jinlu; Zhou, Xiaoping; Zhang, Han; Zhang, Qingyuan
Title: Tumor-associated macrophages secrete CC-chemokine ligand 2 and induce tamoxifen resistance by activating PI3K/Akt/mTOR in breast cancer Document date: 2019_12_19
ID: yj9mb88g_14
Snippet: CCL2 and tumor necrosis factor (TNF)α present in culture supernatants from MCF7-S, MCF7-R, MΦ, MS, and MR macrophages were quantified using specific ELISA kits (Quantikine Human CCL2 Immunoassay and Quantikine Human TNFα Immunoassay; R&D Systems) following the manufacturer's instructions. Concentration of the samples was estimated from the standard curve. CCL2 and TNFα levels below the detection limit of the assay were recorded as zero......
Document: CCL2 and tumor necrosis factor (TNF)α present in culture supernatants from MCF7-S, MCF7-R, MΦ, MS, and MR macrophages were quantified using specific ELISA kits (Quantikine Human CCL2 Immunoassay and Quantikine Human TNFα Immunoassay; R&D Systems) following the manufacturer's instructions. Concentration of the samples was estimated from the standard curve. CCL2 and TNFα levels below the detection limit of the assay were recorded as zero.
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